rabbit anti muscarinic type 1 receptor Search Results


human prox1 antibody  (Bio-Techne corporation)
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Bio-Techne corporation human prox1 antibody
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m 2 machr  (Alomone Labs)
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rabbit anti muscarinic type 1 receptor  (Santa Cruz Biotechnology)
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Rabbit Anti Muscarinic Type 1 Receptor, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit α-muscarinic receptor type 1  (Millipore)
90
Millipore rabbit α-muscarinic receptor type 1
Rabbit α Muscarinic Receptor Type 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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muscarinic acetylcholine receptor type 1  (Millipore)
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Millipore muscarinic acetylcholine receptor type 1
Muscarinic Acetylcholine Receptor Type 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat α-prox1  (R&D Systems)
90
R&D Systems goat α-prox1
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Goat α Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-trpc4 antibody  (Alomone Labs)
93
Alomone Labs anti-trpc4 antibody
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Anti Trpc4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti m 3 -machr  (Alomone Labs)
94
Alomone Labs anti m 3 -machr
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Anti M 3 Machr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-trpc1 antibody  (Alomone Labs)
94
Alomone Labs anti-trpc1 antibody
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Anti Trpc1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibodies for trpc5  (Millipore)
90
Millipore rabbit polyclonal antibodies for trpc5
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Rabbit Polyclonal Antibodies For Trpc5, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calbindin antibody  (Swant)
90
Swant calbindin antibody
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Calbindin Antibody, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neuropeptide y antibody  (ImmunoStar inc)
90
ImmunoStar inc neuropeptide y antibody
Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with <t>PROX1</t> (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).
Neuropeptide Y Antibody, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with PROX1 (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).

Journal: The Journal of Neuroscience

Article Title: Gq-Coupled Muscarinic Receptor Enhancement of KCNQ2/3 Channels and Activation of TRPC Channels in Multimodal Control of Excitability in Dentate Gyrus Granule Cells

doi: 10.1523/JNEUROSCI.1781-18.2018

Figure Lengend Snippet: Stimulation of exogenous Gq-coupled muscarinic DREADD receptors enhances IM in DGGCs. A, Confocal images of hippocampal slices immunostained for HA-tagged hM3Dq receptors (green) and counterstained with PROX1 (red), a cellular marker of DGGCs, from a Cre POMC+ mouse. The hM3Dq-HA tagged fluorescence was present in the molecular (dendrites) and granule (soma) layers of the DG, but not the hilus (mossy fiber axons). CrePOMC+ hM3Dq mice displayed exclusive expression of DREADD receptors to the somatodendritic compartment of DGGCs. The CA1 and CA3 regions did not demonstrate hM3Dq expression. Inset, Confocal micrograph from a CrePOMC− hippocampus negative control demonstrating no hM3Dq receptor expression. B, Fluorescent images show either CrePOMC+hM3Dq-mCitrine fluorescence (top) or CrePOMC− (bottom) granule layer live slices used for electrophysiology recordings obtained using a 488 nm emission filter. C, Summarized current-clamp action potential thresholds of hM3Dq-expressing DGGCs before (ACSF) and after bath-application of CNO (0.1 or 0.3 μm; n = 7 cells, *p < 0.05). D, Bars summarize the enhancement of IM by bath-application of CNO to DGGCs with transgenic insertion of hM3Dq DREADDs (Cre+ DREADD+/−, gray solid bars) specific to DG or in control littermate DGGCs not expressing hM3Dq-DREADDs from mice that lack the Cre gene (Cre− DREADD+/−, striped bars). The dashed line represents mean IM amplitude for ACSF (control) in Cre+ DREADD+/− mice. E, Summarized current-clamp action potential spike frequencies from hM3Dq-expressing DGGCs (n = 7 cells).

Article Snippet: Experiments probing with antibodies rabbit α-muscarinic receptor type 1 (Millipore, RRID: AB_91713 ), goat α-PROX1 (R&D Systems, RRID: AB_2170716 ), and mouse α-HA(12CA5) (Roche Diagnostics, RRID: AB_514505 ) were also conducted.

Techniques: Marker, Fluorescence, Expressing, Negative Control, Transgenic Assay